2039-pT7A3-6H-sso7d-wt-Taq-N

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Cloning of 2039-pT7A3-6H-sso7d-wt-Taq-N

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Purpose: Cloning of 2039-pT7A3-6H-sso7d-wt-Taq-N

Date: 2013-1-23------2013-1-27

Notebook:0

Page:

Clone ID:2039

Experimenters: yingying

Results

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cloning

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PCR N terminus Taq from 462 pTrc-Wt-Taq (from Xu Yan)
wt-Taq-Nde1-F
wt-Taq-HindIII-R

lane
1
 2

 pcr
 1846 pT7A3-6H-sso7d

 Nde1 + HindIII


Colony Screening

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Date: 2014-1-27

Make miniprep and digest with Nde1 + HindIII



Result intepretation/Summary

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Conclusion

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