1965-pCMV5-n-NA-iCre

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Cloning of 1965-pCMV5-n-NA-iCre

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Purpose: Cloning of 1965-pCMV5-n-NA-iCre

Date: 2014-1-27---2014-1-29

Notebook:0

Page:

Clone ID:1965

Experimenters: yingying

Results

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cloning

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PCR iCre from 1961 pCMV5-HTNCre
6His-EcoR1-F
iCre-Eag1-Kpn1-R


lane
 1
 2

 pcr
 1964 pCMV5-n-NA-NLS-Flp-WPRE-oriP

 EcoR1 + Eag1




Colony Screening

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Date: 2014-1-29

Make miniprep and digest with  EcoR1 + Eag1



Result intepretation/Summary

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Conclusion

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