2013-pCMV-FRT-TurboRFP-ODC-2Stops-FRT-TurboGFP

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Revision as of 12:02, 30 January 2014 by Paul.xu (talk | contribs) (Created page with "== '''Cloning of 2013-pCMV-FRT-TurboRFP-ODC-2Stops-FRT-TurboGFP''' == '''Purpose:''' Cloning of 2013-pCMV-FRT-TurboRFP-ODC-2Stops-FRT-TurboGFP '''Date: '''2014-1-27---2014-1-2...")
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Cloning of 2013-pCMV-FRT-TurboRFP-ODC-2Stops-FRT-TurboGFP

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Purpose: Cloning of 2013-pCMV-FRT-TurboRFP-ODC-2Stops-FRT-TurboGFP

Date: 2014-1-27---2014-1-29

Notebook:0

Page:

Clone ID:2013

Experimenters: yingying

Results

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cloning

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PCR FRT-TurboRFP-ODC-2Stop-FRT-TurboGFP from 1922 pLVX-Kozak-FRT-TurboRFP-ODC-2Stop-FRT-TurboGFP
Nhe1-BamH1-Kozak-FRT-F
TurboGFP-HindIII-Sal1-R


lane
 3
 4

 pcr
 1522 CMV-Brainbow-2.1-R

 Nhe1 + Sal1
 Nhe1 + Xho1


Colony Screening

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Date: 2014-1-29

Make miniprep and digest with HindIII + BamH1


c:vector-1522 CMV-Brainbow-2.1-R


Result intepretation/Summary

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Conclusion

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