727-pBAD-sfGFP-C66G
Cloning of 727-pBAD-sfGFP-C66G
[edit]Purpose: Cloning of 727-pBAD-sfGFP-C66G
Date: 2014-1-30---2014-2-3
Notebook:0
Page:
Clone ID:727
Experimenters: yingying
Results
[edit]cloning
[edit]
PCR using pBad24-sfGFP as template
new oligo
sfGFP-C66G-F
Ecoli-sfGFP20
Gel Purify PCR product,
then use the purified PCR fragment as template, run PCR
Ecoli-sfGFP1
Ecoli-sfGFP20
lane
3
4
pcr
545 pBad24
EcoR1 + HindIII
Colony Screening
[edit]Date: 2014-2-3
Make miniprep and digest with EcoR1 + HindIII
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Result intepretation/Summary
[edit]Conclusion
[edit]