1142-pLN6H-Tth-SSB
Cloning of 1142-pLN6H-Tth-SSB
[edit]Purpose: Cloning of 1142-pLN6H-Tth-SSB
Date: 2014-2-12---2014-2-14
Notebook:0
Page:
Clone ID:1142
Experimenters: yingying
Results
[edit]cloning
[edit]
PCR using Tth Genomic DNA from ATCC (-80 oC freezer ATCC box)
TTh-SSB-Nde1-F
TTh-SSB-Xho1-R
| Lane |
3 |
4 |
| PCR |
2029 pLNC6H-sfGFP | |
| Nde1 + Xho1 | ||
Colony Screening
[edit]Date: 2014-2-14
Make miniprep and digest with Nde1 + Xho1
2014-2-18
digest with Sma1+Xho1
c:20292029 pLNC6H-sfGFP
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Result intepretation/Summary
[edit]Conclusion
[edit]