2028 pT7A3-Fast-Kfu
Cloning of 2028 pT7A3-Fast-Kfu
[edit]Purpose: Cloning of2028 pT7A3-Fast-Kfu==
Date: 2014-2----
Notebook:0
Page:
Clone ID:2028
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR
1
Fast-Kfu-SacI-F C10 atggaaggagctcgaagaaaag
fast-Kfu-F591L-R C9 cttcttcgtgacgaaCAGgccgcgtttgtag
2.
fast-Kfu-F591L-F C8 ctacaaacgcggcCTGttcgtcacgaagaag
Fast-Kfu-HindIII-R C11 ttagcaaagcttcaagaaccctc
Join 2 fragments by PCR
Fast-Kfu-SacI-F C10 atggaaggagctcgaagaaaag
Fast-Kfu-HindIII-R C11 ttagcaaagcttcaagaaccctc
digest and clone back into SacI + HindIII digested 1995 pT3A3-Kfu-sso7d 
SacI + HindIII
Colony Screening
[edit]Date: 2014
Make miniprep, digest with SacI + HindIII
