2063 pT7A3E-ChiBD-6H
Cloning of 2063 pT7A3E-ChiBD-6H
[edit]Purpose: Cloning of2063 pT7A3E-ChiBD-6H==
Date: 2014-2----
Notebook:0
Page:
Clone ID:2063
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR CBD chitin binding domain from 1126 pTXB1
CBD-HindIII-Xho1-Kpn1-F D7 tacgac AAGCTT ctcgag ggtacc ggaggaggtgggtctacgacaaatcctggtgtatc
CBD-Sal1-R D8 gctagagtcgacttgaagctgccacaaggcag
digest with HindIII + Sal1,
clone into HindIII + Xho1 digested vector1892 pT7A3E
Colony Screening
[edit]Date: 2014_2_14
Make miniprep, use Kpn1 + EcoRV to find positive clone
