1146-pT7A3E-Alk-PPtase
Cloning of 1146-pT7A3E-Alk-PPtase
[edit]Purpose: Cloning of 1146-pT7A3E-Alk-PPtase
Date: 2014-2-17---2014-2-20
Notebook:0
Page:
Clone ID:1146
Experimenters: yingying
Results
[edit]cloning
[edit]
gene synthesis
pcr1:Pptase1-1 to Pptase1-8
pcr2:Pptase1-9 to Pptase 2-1
pcr3:Pptase2-2 to Pptase2-9
pcr4:Pptase2-10 to Pptase2-17
PCR:pcr1+pcr2+pcr3+pcr4
| lane |
1 |
2 |
| pcr |
1892 pT7A3E | |
| Nde1 + Xho1 | ||
Colony Screening
[edit]Date: 2014-2-20
Make miniprep and digest with Nde1 + Xho1
<br>
Result intepretation/Summary
[edit]Conclusion
[edit]