2079-pLVX-p53

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Cloning of 2079-pLVX-p53

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Purpose: Cloning of 2079-pLVX-p53

Date: 2014-2-19---2014-2-21

Notebook:0

Page:

Clone ID:2079

Experimenters: yingying

Results

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cloning

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PCR from 80 pLex-p53wt
PCR from 1341-pDB.His.MBP-(3C)-p53wt
p53-EcoR1-Nde1-F
P53-Xho1-BamH1-R1


lane
 3
 4

 pcr
 153 pLVX-Plus

 EcoR1 + BamH1


Colony Screening

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Date: 2014-2-21

Make miniprep and digest with EcoR1 + BamH1


<br>

Result intepretation/Summary

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Conclusion

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