PET28-TurboTEV
Genesynthesis of TEV to pET28b
[edit]Purpose: Genesynthesis of TEV to pET28b.
Date: 2011-6-22
Notebook: Haiyan-1
Page:002943
Clone ID: 203.1
Experimenters: Paul, Haiyan
Results
[edit]Subcloning
[edit]Date: 2011-6-22
PCR amplified TEV1-20 and ligate to the EcoRI-SalI digested pET28b
Colony Screening
[edit]Date: 2011-6-22
Use T7 F,R to PCR screening pET28b-TEV (clone #1-10)
File:PET28b-TEV-pcr-06222011.JPGâ
Lane 1-10, 10 colonies pcr amplify
Result intepretation/Summary
[edit]lane 2 and 5 should be good, inoculate to do the minipre
- Sequencing Result:
Conclusion
[edit]Restriction digestion
shows pLentiGP clone #1 may be good,
pLVGP-PKAalpha 3+4 is good
[edit]
Paul.xu 08:23, 15 June 2011 (EST)