2055 pT7A3E-NpuDnaE-ChiBD-6H

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Cloning of 2055 pT7A3E-NpuDnaE-ChiBD-6H

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Purpose: Cloning of2055 pT7A3E-NpuDnaE-ChiBD-6H==

Date: 2014-2----

Notebook:0

Page:

Clone ID:2055

Experimenters:  shanyi

Results

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CLONING

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PCR from 1742 p8H-ELK-IN
NpuDnaE-N-Nde1-F E4 CGTTAA CATATG tgt TTAAGCTATGAAACGGAAATcTTG

NpuDnaE-N-Kpn1-R E5 gtcgtaggtaccATTCGGCAAATTATCAAC

digest with Nde1 +Kpn1



Colony Screening

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Date: 2014

Make miniprep, digest with Nde1 +Kpn1


Result intepretation/Summary

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Conclusion

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