2065 p6HIC
Cloning of 2065 p6HIC
[edit]Purpose: Cloning of2065 p6HIC==
Date: 2014-2----
Notebook:0
Page:
Clone ID:2065
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR using 1743 pIC as template
NpuDnaE-C-Xba1-6H-F1, 1 ul of 10 uM
ctagccTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATAatgaaaatcgaaC
NpuDnaE-C-Xba1-6H-F2 , 1 ul of 1 uM
GATATAatgaaaatcgaaCATCATCATCATCATCAcatgatcaaaATtgccacacgt
NpuDnaE-C-4 , 1 ul of 10 uM
GTCGATCATATGATTAGAAGCGATGAAGCCATTTTTGAGTGCAAAATTATGGTCGCGCTCAAC
digest with Xba1 + Nde1
Colony Screening
[edit]Date: 2014
Make miniprep, digest with Xba1 + Nde1
