2098 pFastBac-Dual-VSVG-RSVGag-FLP

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Cloning of 2098 pFastBac-Dual-VSVG-RSVGag-FLP

Purpose: Cloning of 2098 pFastBac-Dual-VSVG-RSVGag-FLP==

Date: 2014-3----

Notebook:0

Page:

Clone ID:2098

Experimenters: shanyi

Results

CLONING

subclone from 2076 pCMV5-RSV-Gag-NLS-Flp-WPRE-oriP using BamH1 + XbaI to 2100 pFastBac-Dual-VSVG

Colony Screening

Date: 2014

Make miniprep, digest with BamH1 + XbaI

digest with BamHI+EcoRI+XbaI

DIGEST WITH KpnI,WILL GET THREE BANDS

Result intepretation/Summary

Conclusion

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Cloning