2131 pCMV5-Gag-Flpe-IRES-VSVG-WPRE-oriP

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Cloning of 2131 pCMV5-Gag-Flpe-IRES-VSVG-WPRE-oriP =

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Purpose: Cloning of2131 pCMV5-Gag-Flpe-IRES-VSVG-WPRE-oriP==

Date: 2014-3----

Notebook:0

Page:

Clone ID:2131

Experimenters:  shanyi

Results

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CLONING

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 subclone Flpe from 1445 pCMV5-Gag-NLS-Flp-WPRE-oriP using EcoR1 + Not1 to 2113 pCMV5-Gag-TagGFP-IRES-VSVG-WPRE-ori



Colony Screening

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Date: 2014_2_

Make miniprep, digestion with EcoR1 + Not1



Result intepretation/Summary

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Conclusion

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