731-pBad24-sfGFP-T63C
Cloning of 731-pBad24-sfGFP-T63C
[edit]Purpose: Cloning of 731-pBad24-sfGFP-T63C
Date: 2014-3-17---2014-3-18
Notebook:0
Page:
Clone ID:731
Experimenters: yingying
Results
[edit]cloning
[edit]
pcr1:546 pBad24-sfGFP as template
new oligo
sfGFP-T63C-F
old oligo
Ecoli-sfGFP20
pcr2:Gel Purify PCR product,
then use the purified PCR fragment as template, run PCR
Ecoli-sfGFP1
Ecoli-sfGFP20
| lane |
1 |
2 |
| PCR |
545, pBad24 | |
| EcoR1 + HindIII | ||
Colony Screening
[edit]Date: 2014-3-18
Make miniprep and digest with EcoR1 + HindIII
<br>
Result intepretation/Summary
[edit]Conclusion
[edit]
Cloning of 731-pBad24-sfGFP-T63C
[edit]Purpose: Cloning of 731-pBad24-sfGFP-T63C
Date: 2014-3-25---2014-3-27
Notebook:0
Page:
Clone ID:731
Experimenters: yingying
Results
[edit]cloning
[edit]
pcr1:546 pBad24-sfGFP as template
new oligo
new_sfGFP-T63C-F
old oligo
Ecoli-sfGFP20
pcr2:Gel Purify PCR product,
then use the purified PCR fragment as template, run PCR
Ecoli-sfGFP1
Ecoli-sfGFP20
| lane |
1 |
2 |
| PCR |
545, pBad24 | |
| EcoR1 + HindIII | ||
Colony Screening
[edit]Date: 2014-3-27
Make miniprep and digest with EcoR1 + HindIII
<br>
Result intepretation/Summary
[edit]Conclusion
[edit]