Liposome preparation

Liposome by Extrusion

1. dissolve
DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine),
POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine),
LPC(1-stearoyl-2-hydroxy-sn-glycero-3-phosphocholine)
and cholesterol (Avanti Polar Lipids, Alabaster, AL) individually in chloroform.

2. mix lipid components at a molar ratio of 4:4:2 DOPC/POPC/Cholesterol.
3. Dry lipid solutions under vacuum for 3 h and rehydrate in MES buffer to a concentration of 0.5 mg/mL.

4. extrude lipids 10 times through a 50-nm pore size polycarbonate membrane filter (GE HealthcareLifeScience,Pittsburgh,PA) to form vesicles that were ~100 nm in diameter, determined by dynamic light
scattering (Malvern Instruments, Worcestershire, UK)