2136 pCMV5-tat-CD63-iCre

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Cloning of 2136 pCMV5-tat-CD63-iCre

Purpose: Cloning of2136 pCMV5-tat-CD63-iCre==

Date: 2014-4----

Notebook:0

Page:

Clone ID:2136

Experimenters: shanyi

Results

CLONING

subclone tat-CD63 from 2125 pCMV5-tat-CD63-TagGFP to 1968 pCMV5-Gag-p2b-NLS-Cre-WPRE-oriP using Sal1 + EcoR1

Colony Screening

Date: 2014_4_

Make miniprep, digestion with BamH1 + EcoR1

there is one BamHI site in cre, so use BamH1 + EcoR1 to digest ,we will get there bands

Result intepretation/Summary

Conclusion

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Cloning