1841-pCMV5-PKAalpha
Cloning of 2183-pEC-PKAalpha
[edit]Purpose: Cloning of 2183-pEC-PKAalpha
Date: 2014-4-1--2014-4-3
Notebook:0
Page:
Clone ID:2183
Experimenters: yingying
Results
[edit]cloning
[edit]
| 1 |
2 |
3 |
4 | |
| pcr |
1395, pCMV5-hIgG-Lc-TagGFP-Fc(3c)-WPRE-OriP |
pcr |
1454,pEC | |
| Nhe1+Kpn1 | ||||
Colony Screening
[edit]Date: 2014-4-3
Make miniprep and digest with Xho1
6~10:from vector 1454,pEC
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Result intepretation/Summary
[edit]Conclusion
[edit]
Cloning of 1841-pCMV5-PKAalpha
[edit]Purpose: Cloning of 1841-pCMV5-PKAalpha
Date: 2014-4-6---2014-4-9
Notebook:0
Page:
Clone ID:1841
Experimenters: yingying
Results
[edit]cloning
[edit]
| lane |
3 |
4 |
| 2183pEC-PKAalpha |
13951395 pCMV5-hIgG-Lc-TagGFP-Fc(3c)-WPRE-OriP | |
| Nhe1+Kpn1 | ||
Colony Screening
[edit]Date: 2014-4-9
Make miniprep and digest with Xho1
