PET28b-ssfGFP
Gene Synthesis of ssfGFP to pET28b
[edit]Purpose: Gene synthesis of ssfGFP to pET28b
Date: 2011-6-20 to 2011-6-24
Notebook: Robert
Page: 001547
Clone ID: 169
Experimenters: Robert
Results
[edit]Gene Synthesis
[edit]Date: 2011-6-23
Gene synthesis, mkate1 + ssfGFP19, using these mixture: ssfGFP1
ssfGFP2
ssfGFP3
ssfGFP4
ssfGFP5
ssfGFP6
ssfGFP7
ssfGFP8
ssfGFP9
ssfGFP10
ssfGFP11
ssfGFP12
ssfGFP13
ssfGFP14
ssfGFP15
ssfGFP16
ssfGFP17
ssfGFP18
ssfGFP19
Digest BamH1 + XhoI
Ligate to BamH1 + XhoI digested pET28b
Colony Screening
[edit]Date: 2011-6-24
Make miniprep and digest with BamH1 + XhoI
Lanes 1-6: pET28b, BamH1 + XhoI
Result intepretation/Summary
[edit]- Lane 6 has the correct number of bands.
Conclusion
[edit]Restriction digestion shows that clone #6 is good.
Robert.Cai 02:48, 24 June 2011 (EST)