2141-pCMV5-SV40-LargeT-Antigen
Cloning of 2141-pCMV5-SV40-LargeT-Antigen
[edit]Purpose: Cloning of 2141-pCMV5-SV40-LargeT-Antigen
Date: 2014-4-21---2014-4-23
Notebook:0
Page:
Clone ID:2141
Experimenters: yingying
Results
[edit]cloning
[edit]PCR from 413 pCDNA3-YFP-IRES-SVLT
Large-T-BamH1-F
Large-T-EcoR1-R
| lane |
1 |
2 |
| PCR |
1973 pCMV5-CD16-RIgE | |
| BamH1 + EcoR1 | ||
Colony Screening
[edit]Date: 2014-4-23
Make miniprep and digest with BamH1 + EcoR1