2141-pCMV5-SV40-LargeT-Antigen

From ELN
Revision as of 14:33, 23 April 2014 by Paul.xu (talk | contribs) (Created page with "== '''Cloning of 2141-pCMV5-SV40-LargeT-Antigen''' == '''Purpose:''' Cloning of 2141-pCMV5-SV40-LargeT-Antigen '''Date: '''2014-4-21---2014-4-23 '''Notebook:0''' '''Page:'...")
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search

Cloning of 2141-pCMV5-SV40-LargeT-Antigen

[edit]

Purpose: Cloning of 2141-pCMV5-SV40-LargeT-Antigen

Date: 2014-4-21---2014-4-23

Notebook:0

Page:

Clone ID:2141

Experimenters: yingying

Results

[edit]

cloning

[edit]


PCR from 413 pCDNA3-YFP-IRES-SVLT
Large-T-BamH1-F
Large-T-EcoR1-R


lane
1
2

PCR
1973 pCMV5-CD16-RIgE

BamH1 + EcoR1



Colony Screening

[edit]

Date: 2014-4-23

Make miniprep and digest with  BamH1 + EcoR1



Result intepretation/Summary

[edit]

Conclusion

[edit]