2148-pT7A3HC-6H-Tat-sc-PP7

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Cloning of 2148-pT7A3HC-6H-Tat-sc-PP7

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Purpose: Cloning of 2148-pT7A3HC-6H-Tat-sc-PP7

Date: 2014-4-22---2014-4-24

Notebook:0

Page:

Clone ID:2148

Experimenters: yingying

Results

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cloning

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pcr: PP7 from 2147 pT7A3-HC-6H-PP7
1 ul of 10 uM of
GLinker-PP7-F
0.5 ul of 1 uM
PP7-BamH1-F
0.5 ul of 1 uM
PP7-BamH1-F2
1 ul of 10 uM of
PP7-EcoR1-Term-R


Digest with BamH1 + EcoR1

Clone into BamH1 + EcoR1 digested 2147 pT7A3-HC-6H-PP7



Colony Screening

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Date: 204-4-24

Make miniprep and digest with BamH1 + EcoR1


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Result intepretation/Summary

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Conclusion

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