2169-pPIC9k-Enterokinase

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Cloning of 2169-pPIC9k-Enterokinase

Purpose: Cloning of 2169-pPIC9k-Enterokinase

Date: 2014-4-24---2014-4-28

Notebook:0

Page:

Clone ID:2169

Experimenters: yingying

Results

cloning

PCR enterokinase from 1816 pCold-MBP-(E3C)-Enterokinase

enterokinase-EcoR1-F C5
C-6XHis-Nde1-Not1-R


	pcr	2158 pPIC9k
	EcoR1 + Not1

Colony Screening

Date: 2014-4-28

Make miniprep and digest with EcoR1 + EcoRV

<br>

Result intepretation/Summary

Conclusion

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Cloning