2185-pCold-MBP-(Ent-TEV)

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Cloning of 2185-pCold-MBP-(Ent-TEV)

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Purpose: Cloning of 2185-pCold-MBP-(Ent-TEV)

Date: 2014-5-2---2014-5-5

Notebook:0

Page:

Clone ID:2185

Experimenters: yingying

Results

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cloning

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PCR from 1804 pCold-MBP-(E3C)

1. Step 1
Fragment 1
Cold-promoter-Nhe1-F
Cold-pomoter-MBP-R

Fragment 2

Cold-pomoter-MBP-F
MBP-TEV-Nco1-Nde1-R

PCR:pcr1+pcr2


PCR
 1804 pCold-MBP-(E3C)

 Nhe1 + Nde1


Colony Screening

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Date: 2014-5-5

Make miniprep and digest with Nhe1 + Nco1


<br>

Result intepretation/Summary

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Conclusion

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