2223 pFastbacDualM

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Cloning of 2223 pFastbacDualM

Purpose: Cloning of 2223 pFastbacDualM==

Date: 2014-5----

Notebook:0

Page:

Clone ID:2223

Experimenters: shanyi

Results

CLONING

subclone p10-VSVG from 2100 pFastBac-Dual-VSVG using Kpn1 + BamH1 to1979 pUltrabacM

CUT THE SECOND BAND OF 2100 AND THE FIRST BAND OF 1979( UP TO DOWN)

Colony Screening

Date: 2014

Make miniprep, digest with Kpn1 + BamH1

Result intepretation/Summary

Conclusion

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Cloning