1078 1203 1245 1026 1557 1802 1819 1833 1886 1887 1898 1929 1930 1935 1937 1938 1939 1943 2025 2026 2194 2196
Protein Expression
[edit]2014/5/22
SHANYI
| Lane |
Name |
Protein Name |
Clone ID |
Host |
Induction |
SAMPLE |
pI/Mw |
express |
| 1 |
pET28b-scIHF2 |
scIHF2 |
1078 |
15 ml, Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
22.76 |
no |
| 2 |
pET42b-scIHF2 |
scIHF2 |
1203 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
22.76 |
no |
| 3 |
pDB.His.PfuMBP.scIHF2 |
scIHF2 |
1245 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
S |
no | |
| 4 |
pET28b-LambdaExo |
LambdaExo |
1026 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
6.26/28.1 |
|
| 5 |
pT7A3-MBP-(3C)-Lambda-Exo |
MBP-(3C)-Lambda-Exo |
1557 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
5.32/70.5 |
only Lambda-Exo |
| 6 |
pageRuler Plus Prestained protein ladder |
|||||||
| 7 |
pT7A3-Tth-Ligase-K294R |
Tth-Ligase-K294R |
1802 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
6.56/79.1 |
no |
| 8 |
pT7A30-TthDNALigase-K294R-C-6XHis |
TthDNALigase-K294R-C-6XHis |
1819 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
6.43/78.5 |
no |
| 9 |
pT7A3-TRX |
thioredoxin |
1833 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
5.21/17.2 |
yes |
| 10 |
pT7A3-T7-DNA-Polymerase |
T7-DNA-Polymerase |
1886 |
15 ml,
Tuner(DE3)plysN |
0.3mM IPTG RT O/N |
S |
6.24/81.9 |
no |
| 11 |
pT7A3-T7-DNA-Pol-D28 |
T7-DNA-Pol-D28 |
1887 |
15 ml,
Tuner(DE3)plysN |
0.3mM IPTG RT O/N |
S |
6.1/78.9 |
no |
| 12 |
pT7A3-8H-Bst-Exo- |
8H-Bst-Exo- |
1898 |
15 ml,
Nico21(DE3)plysN |
s |
5.86/69 |
yes |
| Lane |
Name |
Protein Name |
Clone ID |
Host |
Induction |
SAMPLE |
pI/Mw |
express |
| 1 |
pT7A30-Ecoli-Ligase |
Ecoli-Ligase |
1929 |
15 ml, Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
5.58/75.3 |
YES |
| 2 |
pT7A3-T3-DNA-Ligase-sso7d-6H |
-DNA-Ligase-sso7d-6H |
1930 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
5.84/48.3 |
no |
| 3 |
pT7A3E-Bst1-Exo-HI |
-Bst1-Exo-HI |
1935 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
S |
5.81/81.2 |
yes |
| 4 |
pT7A3E-DNA-Pol-1 |
DNA-Pol-1 |
1937 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
5.55/105.5 |
yes |
| 5 |
pT7A3E-Klenow(3'->5'_exo–) |
Klenow(3'->5'_exo–) |
1938 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
5.95/70.5 |
yes |
| 6 |
pT7A3E-Klenow |
Klenow |
1939 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
5.84/70.6 |
yes |
| 7 |
pT7A3E-T7-RNAPol-Y639F/H784A |
T7-RNAPol-Y639F/H784A |
1943 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
6.75/100.6 |
yes |
| 8 |
pT7A3E-T4-Ligase-HNH-6H |
T4-Ligase-HNH-6H |
2025 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
6.5/61.7 |
yes |
| 9 |
pT7A3E-T4-Ligase-HI-6H |
T4-Ligase-HI-6H |
2026 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
6.39/69 |
yes |
| 10 |
pageRuler Plus Prestained protein ladder |
|
||||||
| 11 |
pT7A3E-HC-Tth-End-IV |
-HC-Tth-End-IV |
2194 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
S |
6.16/30.6 |
yes |
| 12 |
pT7A3E-PBCV-1-Lig- |
PBCV-1-Lig |
2196 |
15 ml,
Nico21(DE3)plysN |
s |
6.68/34.2 |
yes |