1999 2000 1562 1558 1567 1097 1114 1142 2034 2016
Protein Expression
[edit]2014/6/4
SHANYI use 20ul magnetic beads
| Lane |
Name |
Protein Name |
Clone ID |
Host |
Induction |
SAMPLE |
pI/Mw |
express |
| 1 |
pT7A3E-HC-Exo-VIII |
Exo-VIII |
1999 |
15 ml, Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
4.79/98.1 |
NO, has expression with MBP |
| 2 |
pT7A3-8H-E_coli_Endo IV |
E_coli_Endo IV |
2000 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
5.88/32.9 |
yes |
| 3 |
2000 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
p |
yes | |||
| 4 |
pageRuler Plus Prestained protein ladder |
|||||||
| 5 |
pT7A3-T4-Endonuclease-VII |
T4-Endonuclease-VII |
1562 |
15 ml,
Tuner(DE3)plysN |
0.3mM IPTG RT O/N |
s |
8.96/20.2 |
no expression in both Tuner(DE3)plysN and Nico21(DE3)plysN |
| 6 |
pT7A3-T7-Exo |
T7-Exo |
|
15 ml,
Tuner(DE3)plysN |
0.3mM IPTG RT O/N magnetic beads |
s |
5.37/36.7 |
no expression in both Tuner(DE3)plysN and Nico21(DE3)plysN |
| 7 |
pT7A3-T5-Exo |
T5-Exo |
1567 |
15 ml,
Nico21(DE3)plysN |
s |
5.55/34.5 |
yes | |
| 8 |
pT7A3-T4-G32 |
4 Gene 32 Protein |
1097 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
5.26/35.5 |
|
| 9 |
pLC6H-TneSSB |
TneSSB |
1114 |
15 ml, Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
5.39/18.4 |
yes |
| 10 |
pLN6H-Tth-SSB |
Tth-SSB |
1142 |
15 ml,
Tuner(DE3)plysN |
0.3mM IPTG RT O/N |
S |
5.7/31.1 |
yes |
| 11 |
pLC6H-T7-SSB |
T7-SSB |
2034 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
S |
5.02/27.4 |
yes size is alittle larger. |
| 12 |
pLC6H-Sso-SSB |
Sso-SSB |
2016 |
15 ml,
Tuner(DE3)plysN |
s |
8.08/17.8 |
not sure |