2002 2035 1536 1317 2004 2009 2010 1723 1285
Protein Expression
[edit]2014/6/4
SHANYI use 20ul magnetic beads
| Lane |
Name |
Protein Name |
Clone ID |
Host |
Induction |
SAMPLE |
pI/Mw |
express |
| 1 |
pT7A3E-HC-RnaseH |
RnaseH |
2002 |
15 ml, Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
7.21/19.8 |
NO, has expression with MBP |
| 2 |
pLN6H-Tth-RNase-H |
-Tth-RNase-H |
2035 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
9.54/20 |
yes |
| 3 |
pT7A3-RNH1 |
(Human RNase Inhibitor) |
1536 |
15 ml,
Nico21(DE3)prareplysS |
0.3mM IPTG RT O/N |
s |
5.01/52 |
no expression in both BL21DE3pRare2pLysS and Nico21(DE3)prareplysS |
| 4 |
1536 |
15 ml,
Nico21(DE3)prareplysS |
0.3mM IPTG RT O/N |
p |
||||
| 5 |
pageRuler Plus Prestained protein ladder |
|||||||
| 6 |
pT7A3-RNH1 |
(human ribonuclease/angiogenin inhibitor 1 ) |
|
15 ml,
Nico21(DE3)prareplysS |
0.3mM IPTG RT O/N magnetic beads |
s |
4.97/51.9 |
no expression in both BL21DE3pRare2pLysS and Nico21(DE3)prareplysS |
| 7 |
|
1317 |
15 ml,
Nico21(DE3)prareplysS |
p |
|
|||
| 8 |
pT7A3E-HC-RNaseIII |
RNaseIII |
2004 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
6.54/27.3 |
YES |
| 9 |
pL42-RNaseA |
RNaseA |
2009 |
15 ml, Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
s |
8.42/15 |
NO, has expresion in pT7A3 vector |
| 10 |
pL42-RNaseT1 |
RNaseT1 |
2010 |
15 ml,
Nico21(DE3)plysN |
0.3mM IPTG RT O/N |
S |
5.55/25.1 |
no |
| 11 |
pT7A3-6His-VVCap-S-GGGS |
VVCap-S-GGGS |
1723 |
15 ml,
Tuner(DE3)plysN |
0.3mM IPTG RT O/N |
S |
8.67/39 |
no |
| 12 |
pT7A3-Proteinase-K |
Proteinase-K |
1285 |
15 ml,
Tuner(DE3)plysN |
s |
7.05/44.1 |
no expression in both Nico21(DE3)plysN and Tuner(DE3)plysN |
need to redo
pL42 vector: induce at 42 oC for 2~3 hours, grow at 30 oc