2243 pEC2-Exo-VII
Cloning of 2243 pEC2-Exo-VII
[edit]Purpose: Cloning of 2243 pEC2-Exo-VII
Date: 2014-6
Notebook:0
Page:
Clone ID:2243
Experimenters: shanyi
Results
[edit]cloning
[edit]PCR from E. Coli K12 substr. MG1655 genomic DNA
Ecoli-Exo-VII-Nde1-F gct cac CATatgttaccttctcaatcccctg
Ecoli-Exo-VII-Xho1-R gctggcCTCGAGttaatgcacctttttacgcgat (atcgcgta aaaaggtgca ttaa CTCGAG gccagc)
digest with NdeI+XhoI
Colony Screening
[edit]Date: 2014-6
mini prep and digest with NdeI+XhoI
Result intepretation/Summary
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Conclusion
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