2224 pFastbacDual-p10-VSVG-M
Cloning of 2224 pFastbacDual-p10-VSVG-Me
[edit]Purpose: Cloning of 2224 pFastbacDual-p10-VSVG-M
Date: 2014-6
Notebook:0
Page:
Clone ID:2224
Experimenters: shanyi
Results
[edit]cloning
[edit]PCR VSVG from 505 pCMV-VSVG
1st fragment VSVG-Nco1-F ctcatttccatggacatcac VSVG-dKpn1-R gtattttagggtGccattgattatggtg
2nd fragment VSVG-dKpn1-F caccataatcaat ggC acc ctaaaatac VSVG-Kpn1-R A4 gtgtaggtaccttactttccaagtcggttcatc
3. Purify on gel 4. Join them by PCR
5. digest with Nco1 + Kpn1
6. clone back to vector 2223 pFastbacDualM
Colony Screening
[edit]Date: 2014-6
mini prep and digest with Nco1 + Kpn1
Result intepretation/Summary
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Conclusion
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