2112 pFastBac-Dual-VSVG-HIVGag-iCre-CMV-iCre

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Cloning of 2112 pFastBac-Dual-VSVG-HIVGag-iCre-CMV-iCre

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Purpose: Cloning of 2112 pFastBac-Dual-VSVG-HIVGag-iCre-CMV-iCre

Date: 2014-6

Notebook:0

Page:

Clone ID:2112

Experimenters: shanyi

Results

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subcloning

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subclone CMV-NLS-Cre from 2239 pAAV-CMV-iCre using Not1 + HindIII to 2099pFastBac-Dual-VSVG-HIV-Gag-Cre

2239digest with Not1 + Xba1+BsaI,CUT the first band




Colony Screening

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Date: 2014-6

mini prep and digest with EcoR1


verify using Not1 + Xba1 and BamHI




Result intepretation/Summary

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  • <br>

Conclusion

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<br>





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