Cre VLP Activity assay 20140509

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= Making VLP
= 2123 pCMV5-Mfge8-Tat-Cre-MFGE8 + /- 1968 pCMV5-Gag-p2b-NLS-Cre-WPRE-oriP +/-VSVG
2119 pCMV5-CD63-iCre + /- 1968 pCMV5-Gag-p2b-NLS-Cre-WPRE-oriP +/-VSVG
2138 pCMV5-pene-CD63-iCre + /- 1968 pCMV5-Gag-p2b-NLS-Cre-WPRE-oriP +/-VSVG
2136 pCMV5-tat-CD63-iCre + /- 1968 pCMV5-Gag-p2b-NLS-Cre-WPRE-oriP +/-VSVG
2172 pCMV5-CD16-Tat-iCre-RIgE + /- 1968 pCMV5-Gag-p2b-NLS-Cre-WPRE-oriP+/-VSVG

= Activity assay
= add 0.5 ml condition culture medium to 24 well plate, 293T cells transfected with 2181
3 days after adding medium



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