PlentiGP-LoxP

Cloning of LoxP to pLentiGP

Purpose: cloning of LoxP to pLentiGP

Date: 2011-6-28 to 2011-6-29

Notebook:Ruili Su

Page:002788--002789

Clone ID:# 259

Experimenters:  Ruili

PCR

Date: 2011-6-28

PCR to amplify LoxP

1. Marker

2. Amplified LoxP fregment from PCR.

Subcloning

Digest PlentiGP (clone 219) with BamH1+ XHoI

Digest LoxP fregment with BamH1+ XHoI

cut out insert and vector, ligate and transform STBL3

1. Marker.

2. Digested PlentiGP (clone 219) with BamH1+ XHoI.

3. Digested LoxP fregment with BamH1+ XHoI.

Result intepretation/Summary

  

Conclusion

--Ruili.su 14:37, 28 June 2011 (EST)