PTr2-CB-SMN1

Cloning of SMN1 to PTr2-CB

Purpose: cloning of SMN1 to PTr2-CB

Date: 2011-6-24

Notebook: Ruili Su

Page: 002785--002786

Clone ID: 255

Experimenters: Ruili

Results

Gene Synthesis

Date: 2011-6-24

Gene Synthesis oligos (SMN1 throuth SMN12)

<Picture 1>

1. Marker;

3. Synthesized gene from PCR (10 ul synthesized gene).

Subcloning

Date: 2011-6-24

Digest pTr2-CB (244) with HindIII + XhoI

Digest the synthesized gene with HindIII + XhoI

cut out insert and vector, ligate and transform STBL3

<Picture 2>
1. The digested vector PTr2-CB (244)

2 through 4. the digested synthesized gene (404bp)

5. Marker.

Date: 2011-6-27

<Picture 3>.

1. Marker.

2. Digested pAAV-GFP (247)  with EcoRI + Xhol.

3. Digested PTr2-CB(244)with HindIII.

4. Digested PTr2-CB(244)with XHoI.

5. Digested PTr2-CB(244) with HindIII + XHoI (Fast digestion buffer).

6. Digested PTr2-CB(244) with HindIII + XHoI (NEB Buffer).

7. Undigested PTr2-CB(244)

<Picture 4>

1. Marker.

2---7 Samples have nothing to do with this cloning.

8. Digested PTr2-CB(244) with HindIII + XHoI (Fast digestion buffer).

Result intepretation/Summary

• 
  *Sequencing Result:<br>

Conclusion

Restriction digestion <br>

<br>

<br>

--Ruili.su 13:34, 24 June 2011 (EST)