2246-pT7A3E-Lambda-Xis
Cloning of 2246-pT7A3E-Lambda-Xis
[edit]Purpose: Cloning of 2246-pT7A3E-Lambda-Xis
Date: 2014-6-24---2014-6-26
Notebook:0
Page:
Clone ID:2246
Experimenters: LIANGHUO
Results
[edit]cloning
[edit]PCR from 1149 pT7A3E-6H-HC-Xis or 1186 pDB.His.MBP-Xis
Xis-Nde1-F
tagca ctagc cat atgtacttgacacttcaggag
Xis-Xho1-F
(gaaatgggaagaaggcgaagtca ctcgag tagca)
tgctactcgagtgacttcgccttcttcccatttc
2246 DIGESTED
Colony Screening
[edit]Date: 2014-6-26
Make miniprep and digest with Nde1 + XhoI
SELECT CLONE1 FOR SEQUENCING
Result intepretation/Summary
Conclusion
[edit]