2247-pT7A3E-Int
Cloning of 2247-pT7A3E-Int
[edit]Purpose: Cloning of 2247-pT7A3E-Int
Date: 2014-7-01---2014-7-02
Notebook:0
Page:
Clone ID:2247
Experimenters: LIANGHUO
Results
[edit]cloning
[edit]PCR from lambda DNA or 406 pET28b-Int
Int-Nde1-F tacga catatgggaagaaggcgaagtcatg
Int-Xho1-R
(gtgggacaaaattgaaatcaaactcgag tagca)
tgctactcgagtttgatttcaattttgtcccac
2247 DIGESTED
[edit]
Colony Screening
[edit]Date: 2014-07-02
Make miniprep and digest with Nde1 + XhoI
SELECT CLONE3 FOR SEQUENCING
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Result intepretation/Summary
[edit]Conclusion
[edit]