2245-pT7A3E-OmpA-Lambda-Exo
Cloning of 2245-pT7A3E-OmpA-Lambda-Exo
[edit]Purpose: Cloning of 2245-pT7A3E-OmpA-Lambda-Exo
Date: 2014-7-01---2014-7-02
Notebook:0
Page:
Clone ID:2245
Experimenters: LIANGHUO
Results
[edit]cloning
[edit]PCR from lambda DNA or clone 1026 pET28b-LambdaExo
1 ul of 1 uM OmpA-Xba1-F
tagcTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATAatgaaaaaGacCgcgatCgcgattgcAgtggcGCTG
1 ul of 1 uM OmpA-lambdaExo-F
atCgcgattgcAgtggcGCTGgcTggTttCgcGaccgtggcgcaggcgcatatgacaccggacattatcctgcag
1 ul of 10 uM LambdaExo-Xho1-R2
(gtatttggggagcaatggcga ctcgagcgatct)
agatcgctcgagtcgccattgctccccaaatac
2245 DIGESTED
[edit]
Colony Screening
[edit]Date: 2014-7-02
Make miniprep and digest with Xba1 + XhoI
SELECT CLONE3 FOR SEQUENCING
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Result intepretation/Summary
[edit]Conclusion
[edit]