1910-pT7A3E-BsuPol
Cloning of 1910-pT7A3E-BsuPol
[edit]Purpose: Cloning of 1910-pT7A3E-BsuPol
Date: 2014-7-01---2014-7-02
Notebook:0
Page:
Clone ID:1910
Experimenters: LIANGHUO
Results
[edit]cloning
[edit]1. PCR from genomic DNA
Fragment 1.
BsuPol-Nde1-F
ttaaacatatggatcttggcttcaacacgcttcttg
BsuPol-R2 caacgtcgtcataagaattgccc
Fragment 2
BsuPol-F2 gggcaattcttatgacgacgttg
BsuPol-Xho1-R
(catcttggtacgatgcgaaa ctcgag agaatc)
gattctctcgagttatttcgcatcgtaccaagatg
2. Join two fragments
BsuPol-Nde1-F + BsuPol-Xho1-R
3. Purify and digest with Nde1 + Xho1
1910 DIGESTED
[edit]
Colony Screening
[edit]Date: 2014-7-02
Make miniprep and digest with Nde1 + XhoI
SELECT CLONE2 FOR SEQUENCING
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Result intepretation/Summary
[edit]Conclusion
[edit]