Synthesis Chitosan PEI

Reagents

Chitosan: Mw = 50 kDa and deacetylation degree = 90%

1,19-carbonyldiimidazole

Copolymer of CP was prepared according to the methods described by Gao.

Dissolve 0.5 g CS in 20 ml of a 0.5% acetic acid solution

adjust to pH 7.0

Stir the mixture overnight.

Add CDI at a molar ratio of CDI:CS amine = 2:1

Stir for 1 h.

Add PEI (1.8 kDa) dropwise into the solution under stirring at a molar ratio of PEI:amine = 2:1.

Polymerize at room temperature (25 oC) overnight.

Dialyse in water for 48 h.

Lyophilize