2186-pT7A3-HC-R9-HA
Cloning of 2186-pT7A3-HC-R9-HA
[edit]Purpose: Cloning of 2186-pT7A3-HC-R9-HA
Date: 2014-4-24---2014-4-28
Notebook:0
Page:
Clone ID:2186
Experimenters: yingying
Results
[edit]cloning
[edit]
PCR: template2180
R9-HA-1 B10
HA-Xho1-R
| PCR |
1885 pT7A3-HC | |
| Nco1 + Xho1 | ||
Colony Screening
[edit]Date: 2014-4-28
Colony screening by PCR using pET-seq-F and
HA-Xho1-R
<br>
Result intepretation/Summary
[edit]Conclusion
[edit]
Cloning of 2186-pT7A3-HC-R9-HA
[edit]Purpose: Cloning of 2186-pT7A3-HC-R9-HA
Date: 2014-7
Notebook:0
Page:
Clone ID:2186
Experimenters: shanyi
Results
[edit]cloning
[edit]PCR: template2180
R9-HA-1 B10
HA-Xho1-R
digest with NcoI+XhoI, clone into 1885 pT7A3-HC
Colony Screening
[edit]Date: 2014_2_14
Make miniprep, use Nco1 + Xho1 to find positive clone
