2279 pLIKE-rep-T7-Endo1

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Cloning of 2279 pLIKE-rep-T7-Endo1

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Purpose: Cloning of 2279 pLIKE-rep-T7-Endo1==

Date: 2014-7----

Notebook:0

Page:

Clone ID:2279

Experimenters:  shanyi

Results

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CLONING

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PCR from 1225 pET28b-T7-Endo1

T7-endo1-Xba1-F tacta tctaga catatgg caggttacgg cgctaaag

T7-endo1-HindIII-R (taaaaaggaa aggaggaaag aaa ctcgag GGT ACC CACCACCACCACCACCACTGA aagctt tagca) tgctaaagcttTCAGTGGTGGTGGTGGTGGTGGGTACCctcgagtttctttcctcctttccttttta



digest with Xba1 +HindIII, clone to 2270 pLIKE-rep




Colony Screening

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Date: 2014_2_14

Make miniprep, use  Xba1 +HindIII to find positive clone


SEQUENCED NOT RIGHT





Result intepretation/Summary

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Conclusion

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