2268 pHCMC04-iFLP

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Cloning of 2268 pHCMC04-iFLP

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Purpose: Cloning of2268 pHCMC04-iFLP==

Date: 2014-7----

Notebook:0

Page:

Clone ID:2268

Experimenters:  shanyi

Results

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CLONING

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PCR from 2115

6His-BamH1-F tactaggatccatgggccatcaccatcaccatcac

iFLP-Xma1-R (catcaatcgtcgcatctaa ctcgag cccggg tactaa) ttagtacccgggctcgagttagatgcgacgattgatg


digest with BamH1+ Xma1, clone to 2271 pHCMC04



Colony Screening

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Date: 2014_7

Make miniprep, use XhoI to find positive clone



use BamH1+ Xma1  to verify


Result intepretation/Summary

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Conclusion

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