2249 pT7A3E-T4-RNA-Lig2

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Cloning of 2249 pT7A3E-T4-RNA-Lig2

Purpose: Cloning of2249 pT7A3E-T4-RNA-Lig2D==

Date: 2014-7----

Notebook:0

Page:

Clone ID:2249

Experimenters: shanyi

Results

CLONING

PCR from 1123 pT7A3-MBP-(3C)-T4-RNA-Lig2 using

T4RNA-lig-Nde1-F atcgac CATatg tttaaaaagtatagcagtc

T4-RNA-Lig2-Xho1-R2

digest with Nde1 + XhoI, clone to 1892

Colony Screening

Date: 2014_7

Make miniprep, use Nde1 + XhoI to find positive clone

Result intepretation/Summary

Conclusion

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Cloning