2049 pEB-N6H
Cloning of 2049 pEB-N6H
[edit]Purpose: Cloning of2049 pEB-N6H==
Date: 2014-7----
Notebook:0
Page:
Clone ID:2049
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR pBad promoter 2048
using
pBad-Age1-F AACCAAACCGGTAACCCCGCTTAT
pBad-R2 (GTTTTTTTGGGCTAACAGGAGGAATTAAccatgGGTtctAGCCATCATCATCATCATcatatgGGtaCCtagcac) gtgctaGGtaCCcatatgATGATGATGATGATGGCTagaACCcatggTTAATTCCTCCTGTTAGCCCAAAAAAAC
Digest with Age1 + Nde1 Clone into Age1 + Nde1 digested vector
Colony Screening
[edit]Date: 2014_7
Make miniprep, use Age1 + Nde1 to find positive clone
