2287 pL6H-5H-Tat-5H-sso7d
Cloning of 2287 pL6H-5H-Tat-5H-sso7d
[edit]Purpose: Cloning of2287 pL6H-5H-Tat-5H-sso7d==
Date: 2014-7--2014/8/1--
Notebook:0
Page:
Clone ID:2287
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR using 1847 pT7A30-sso7d-6H as template
1 ul of 10 uM 2287-6H-Tat-5H-Nco1-F1 ATC ACC CAT GGG CAG CAG CCA TCA TCA TCA TCA TCA CGG TCG CAA GAA ACG TCG CCA ACG TCG CCG TCC GCC TGC AGG C
1 ul of 1 uM 2287-6H-Tat-5H-F2 TCG CCG TCC GCC TGC AGG CCA CCA TCA CCA TCA CGG TAC CGG TAG CGG CGG TGG TGG CGC CAC GGT CAA ATT CAA GTA C
1 ul of 10 uM 2287-sso7d-Xho1-R TGA TAC TCG AGT TAG CAT TTT TTC TGC TTC TCA AGC ATT TG
digest with Nco1 +Xho1,clone into 1997 pL6H
Colony Screening
[edit]Date: 2014_8_1
Make miniprep, use Nco1 +Xho1 to find positive clone
digest with NcoI+KpnI,will get a 94bp band
