2197 pT7A3-6XHis-Sumo
Cloning of 2197 pT7A3-6XHis-Sumo
[edit]Purpose: Cloning of2197 pT7A3-6XHis-Sumo==
Date: 2014-7----
Notebook:0
Page:
Clone ID:2197
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR from 2285 pBY011-SMT3
SUMO-Nco1-F
tcgac cc atg ggc agc agc cac cat cac cat cac catatgtcggactcagaagtcaatc
SUMO-Nde1-R
(cacagagaacagattggtggt cat atg tagcac)
gtgctacatatgaccaccaatctgttctctgtg
Digest withNco1+ Nde1 Clone into 1279
Colony Screening
[edit]Date: 2014_7
Make miniprep, useNco1+ Nde1 to find positive clone
Cloning of 2197 pT7A3-6XHis-Sumo
[edit]Purpose: Cloning of2197 pT7A3-6XHis-Sumo==
Date: 2014-8----18
Notebook:0
Page:
Clone ID:2197
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR from 2285 pBY011-SMT3
2197-SUMO-Nco1-F1 tcgac cc atg ggc agc agc cac cat cac cat cac cat tcggactcagaagtcaatc
SUMO-Nde1-R
(cacagagaacagattggtggt cat atg tagcac)
gtgctacatatgaccaccaatctgttctctgtg
Digest withNco1+ Nde1 Clone into 1279
Colony Screening
[edit]Date: 2014_9
Make miniprep, useXba1+ Nde1 to find positive clone
