2214 pET30b-Dest-Avi-8H-BirA

From ELN
Revision as of 12:41, 26 August 2014 by Excellgen (talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search

Cloning of 2214 pET30b-Dest-Avi-8H-BirA

[edit]

Purpose: Cloning of2214 pET30b-Dest-Avi-8H-BirA==

Date: 2014-8----

Notebook:0

Page:

Clone ID:2214

Experimenters:  shanyi

Results

[edit]

CLONING

[edit]

PCR from BL21 genomic DNA using these 3 oligos:



1ul of 10 uM of BirA-HindIII-F1 (8_21_2013 A03)
atcgacAAGCTTGCGGCCGCActcgagggactgaacgatatttttgaggcgcaaaaaattgagtggcatgaaCAC

1ul of 1 uM of BirA-F2 (8_21_2013 A04)
attgagtggcatgaaCACCACCACCACCACCACTAACTTTAAGAAGGAGTCTCTCCCATGAAGGATAACACCGTGCCAC


1ul of 10 uM of BirA-Sal1-R (8_21_2013 A05)
(CTGCGTAGTGCAGAAAAATAA gtcgac cgatca)
tgatcggtcgacTTATTTTTCTGCACTACGCAG


cut 1 kb PCR product with HindIII + Sal1,
clone into HindIII + Xho1 digested 2213 pET30b-Dest




Colony Screening

[edit]

Date: 2014_8_

Make miniprep, use EagI to find positive clone

22





Result intepretation/Summary

[edit]

Conclusion

[edit]
Retrieved from "https://eln.excellgen.com/index.php?title=2214_pET30b-Dest-Avi-8H-BirA&oldid=12958"