2232 pT7A3-SortaseAM-C6H
Cloning of 2232 pT7A3-SortaseAM-C6H
[edit]Purpose: Cloning of2232 pT7A3-SortaseAM-C6H==
Date: 2014-8----
Notebook:0
Page:
Clone ID:2232
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR using 2230 pT7A3-SortaseAM-N6H as template
1 ul of 10 uM SortaseA-Nde1-F1 tcgaacatatgGGCAGCAGCctcgagcttccggaaaccggaggaggtgggtctggaggtggaggatctggt 1 ul of 1 uM SortaseA-Nde1-F2 cggaaaccggaggaggtgggtctggaggtggaggatctggtggaggtgggtctCAAGCTAAACCTCAAATTCCG
1 ul of 10 uM SortaseA-Sal1-R tgctagtcgaccttgacttcAgtagctacaaag
digest with Nde1 + Sal1. Digest vector with Nde1 + Xho1
Colony Screening
[edit]Date: 2014_8_
Make miniprep, use NdeI+HindIII to find positive clone
