2300 pEC-wtSM2-b

Cloning of 2300 pEC-wtSM2-b

Purpose: Cloning of2300 pEC-wtSM2-b==

Date: 2014-8----

Notebook:0

Page:

Clone ID:2300

Experimenters:  shanyi

Results

CLONING

PCR using new oligos, 1680 pCold-GST-wtSM2 as template

1 ul of 10 uM SM2-signal-Xba1-F1
tagcTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATAATGCGCTTTAACAACAAGAATGTTGGCCTTG
1 ul of 1 uM SM2-signal-Nde1-F2
ACAAGAATGTTGGCCTTGGCGCCCTGCTGTTCGCCGCGCAGGCGTCGGCCgaccatatgacgctcgaatccatcgac

old oligo
1 ul of 10 uM WtSM2-Xho1-R
(gagttgatgggctgcaaaaac taa ctcgag ctagca)
tgctagctcgagttagtttttgcagcccatcaactc

digest with Xba1 + Xho1, clone to2015

Colony Screening

Date: 2014_8_

Make miniprep, useXba1 + Xho1  to find positive clone

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