212 pL6H-TthMuts
Cloning of 212 pL6H-TthMuts
[edit]Purpose: Cloning of212 pL6H-TthMuts==
Date: 2014-8----
Notebook:0
Page:
'Clone ID:212
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR from HB8 genomic DNA using
PCR using Tth Genomic DNA, digestion, ligation Tth-MutS-F ATC GCA TAT GCT CAA GGG CGA AGG CCC NdeI Tth-MutS-R (CTGGGTAGCATGAAGGGG TGA GAATTCATCG) CGA TGA ATT CTC ACC CCT TCA TGC TAC CCA G
digest with Nde1 + EcoR1, clone to1997
Colony Screening
[edit]Date: 2014_8_
Make miniprep, use Nde1 + EcoR1 to find positive clone
digest with Nde1 + EcoR1+BamHI
