212 429 2238 2197 2249 2287 2232 2291 2290 2294
Protein Expression
[edit]2014/9/5
| Lane |
Name |
Protein Name |
Clone ID |
Host |
Induction |
SAMPLE |
pI/Mw |
express |
| 1 |
pL6H-TthMuts |
TthMuts |
212 |
15 ml, Nico21(DE3)plysN |
42oC O/N |
s |
5.87/91.5 |
yes |
| 2 |
pHisMBP-TVMV |
TVMV |
429 |
15 ml,
Nico21(DE3)plysN |
0.4mM IPTG RT O/N |
s |
7.74/27.4 |
yes |
| 3 |
pT7A3-uvrD |
uvrD |
2238 |
15 ml,
Nico21(DE3)plysN |
0.2mM IPTG RT O/N |
s |
5.98/83.1 |
no |
| 4 |
pT7A3-6XHis-Sumo |
Sumo |
2197 |
15 ml,
Nico21(DE3)plysN |
0.2mM IPTG RT O/N |
6.07/16.1 |
yes | |
| 5 |
pageRuler Plus Prestained protein ladder |
|||||||
| 6 |
pT7A3E-T4-RNA-Lig2 |
T4-RNA-Lig2 |
|
15 ml,
Nico21(DE3)plysN
|
0.2mM IPTG RT O/N magnetic beads |
s |
6.1/39.3 |
yes |
| 7 |
pL6H-5H-Tat-5H-sso7d |
5H-Tat-5H-sso7d |
2287 |
15 ml,
Nico21(DE3)plysN |
42oC O/N |
s |
10.29/11.4 |
cell lysed |
| 8 |
pT7A3-SortaseAM-C6H |
SortaseAM-C6H |
2232 |
15 ml,
Nico21(DE3)plysN |
0.2mM IPTG RT O/N |
s |
6.17/19.7 |
no |
| 9 |
pT7A3-SortaseAM-N6H |
SortaseAM-N6H |
2291 |
15 ml, Nico21(DE3)plysN |
0.2mM IPTG RT O/N |
s |
6.93/22.5 |
yes |
| 10 |
pT7A3-T7-DNA-helicase |
T7-DNA-helicase |
2290 |
15 ml,
Nico21(DE3)plysN
|
0.2mM IPTG RT O/N |
S |
5.66/64.1 |
|
| 11 |
pET28b-T7-DNA-helicase |
T7-DNA-helicase |
2294 |
15 ml,
Nico21(DE3)plysN |
0.4mM IPTG RT O/N |
S |
5.66/64.1 |
not sure |
| 12 |